PTU - Polskie Towarzystwo Urologiczne

NABŁONEK PRZEJŚCIOWY IN SITU I PERSPEKTYWY JEGO HODOWLI IN VITRO DLA CELÓW REPARACYJNYCH
Artykuł opublikowany w Urologii Polskiej 1999/52/4.

autorzy

Krzysztof H. Włodarski, Paweł Wlodarski, Bolesław Kuzaka
Zakład Histologii i Embriologii Instytutu Biostruktury (KHW, PKW) AM w Warszawie
Kierownik: prof. dr hab. S. Moskalewski
Klinika Urologiczna (BK) AM w Warszawie
Kierownik: prof. dr hab. A. Borkowski

słowa kluczowe

układ narządów moczowych hodowla nabłonka przejściowego

streszczenie

Przedstawiono morfologię i cytofizjologię oraz opisano metodę hodowli
ludzkiego nabłonka dróg moczowych in vitro. Hodowle tych komórek na
biodegradowalnych' podłożach (polimery kwasu poliglikolowego, prepara-
ty bezkomórkowej skóry) mogą być wyzyskane do rekonstrukcji w obrębie
dróg moczowych.

piśmiennictwo

  1. [1] Allen, G. R, Bryans, J. T.: Studies of an established equine cell line derwedfrom
  2. transitional cell carcinoma. Am. J. Vet. Res. 1974,35,1153-1160.
  3. [2] Alroy, J., Merk, F. B., Morre, D. ]., Weinstein, R. S.: Membrane differentiation
  4. in the Golgi apparatus ofmammalian urinary bladder epithelium. Anat. Rec. 1982,
  5. 203, 429-440.
  6. [3] Alroy, ]., Goyal, V., Ucci, A. A., Klauber, G. T., Heaney, J. A., Cohen, S. M.:
  7. Cell surface coat of human and rat bladder urothelium. I. Ruthenium-red studies in
  8. non-neoplastic and neoplastic cells. Virchows Arch. B (Cell Pathol.) 1983,42,251-
  9. 262.
  10. [4] Atala, A.: Commentary on the replacement of urologic associated mucosa. J. Urol.
  11. 1996, 156, 338-339.
  12. [5] Atala, A., Vacanti, J. R, Peters, C. A., Mandell, J., Retik, A. B., Freeman
  13. A. R.: Formation of urothelial structures in vivo from dissociated cells attached to
  14. biodegradable polymer scaffolds in vitro. J. Urol. 1992,148, 658-662.
  15. [6] Atala, A., Freeman, M. R., Vacanti, J. R, Shepard, }., Retik, A. B.: Implan-
  16. tation in vivo and retrieval of artificial structures consisting of rabbit and human
  17. urothelium and human bladder muscle. J. Urol. 1993,150, 608-612.
  18. [7] Candiotti, A., Ibanez, N., Monis, B.: Carbohydrate content of transitional epi-
  19. thelium of the urinary system. Experient. 1971, 27, 551-552.
  20. [8] Cilento, B. G., Freeman, M. R., Schenck, F. X., Retik, A. B., Atala, A.:
  21. Phenotypic and cytogenetic characterization of human bladder urothelia expanded in
  22. vitro. J. Urol. 1994,152, 665-670.
  23. [9] DeBoer, W. I., Rebel, J. M. }., Foekens, J. A., Vermey, M., Van der Kwast,
  24. T. H.: Characterization of mouse urothelial cell lines in different phases of transition-
  25. al-cell carcinogenesis. Int. J. Cancer. 1993, 54,1022-1027.
  26. [10] De la Pena, A., Oliveros, M., Tamames, J. M., Mintz, S.: Neoplastic and
  27. malignant changes of the urothelium. Br. J. Urol. 1959,31,473.
  28. [11] Firth, J. A., Hicks, R. M.: Interspecies variation in the fine structure and enzyme
  29. cytochemistry ofmammalian transitional epithelium. J. Anat. 1973,116, 31-43.
  30. [12] Fukushima, S., Arai, M., Cohen, S. M., Jacobs, J. B., Friedell, G. H.: Scan-
  31. ning electron microscopy of cyclophosphanmide-induced hyperplasia of the rat uri-
  32. nary bladder. Lab. Investig. 1981, 44, 89-96.
  33. [13] Gustafson, C. J., Eldh, J., Kratz, G.: Culture of human urothelial cells on a cell-
  34. free dermis for autotransplantation. Eur. Urol. 1998, 33, 503-506.
  35. [14] Holstein, A. F., Sandmann, }., Bressel, ML, Davidoff, M. S.: Reinvestiga-
  36. tion of the transitional epithelium (urothelium) of human ureter. Ann. Anat. 1994,
  37. 176, 109-117.
  38. [15] Hoyes, A. D., Ramus, N. L, Martin, B. G. H.: Fine structure of the epithelium
  39. of the human fetal bladder. J. Anat. 1972,11, 415-425.
  40. [16] Hutton, K. A. R., Trejdosiewicz, L. K., Thomas, D. F. M., Southgate, J.:
  41. Urothelial tissue culture for bladder reconstruction: an experimental study. J. Urol.
  42. 1993 , 150, 721-725.
  43. [17] Jacob, J., Ludgate, C. M., Forde, J., Tulloch, W.: Recent obserations on the
  44. ultrastructure of human urothelium. 1. Normal bladder of elderly subjects. Cell
  45. Tiss. Res. 1978,193, 543-560.
  46. [18] Jost, S. P., Gosling, J. A., Dixon, J. S.: The morphology of normal bladder
  47. urothelium. J. Anat. 1989,167,103-115.
  48. [19] Kirkels, W. J., Pelgrim, O. E., Debruyne, F., Vooijs, G. R, Herman, C. J.:
  49. Soft agar culture of human transitional cell carcinoma colonies from urine. Am. J.
  50. Clin. Pathol. 1982, 78, 690-694.
  51. [20] Lewis, S. A., de Moura, L. C: Incorporation of cytoplasmic vesisles into apical
  52. membrane ofmammalian urinary bladder epithelium. Nature. 1982,297,685-688.
  53. [21] Mende, T., Chambers, E.: Distribution of mucopolisacharide and alkaline phos-
  54. phatase in transitional epithelium.}. Histochem. Cytochem. 1957,5,99.
  55. [22] Minsky, B. D., Chlapowski, F. }.: Morphometric analysis of the translocation of
  56. lumenal membrane between cytoplasm and cell surface of transitional epithelial cells
  57. during the expansion-contraction cycles ofmammalian urinary bladder. J. Cell Biol.
  58. 1978, 77, 685-697.
  59. [23] Monis, B., Zambrano, D.: Ultrastructure of transitional epithelium of man. Ztsch
  60. Zellforsch. 1968, 87, 101-117.
  61. [24] Ostrowski, K., Kieler, ]., Balslev, I.: Differences in adhesion of urothelial cells to
  62. a panel of substrates are characteristic for established human urothelial cell lines
  63. differing in their transformation grade. Anticancer Res. 1990,10, 359-368.
  64. [25] OToole, C. M., Tiptaft, R. C, Stevens, A.: HLA antigen expression on urothe-
  65. lial cells: detection by antibody-dependent cell-mediated cytotoxicity. Int. J. Cancer.
  66. 1982, 29, 391-395.'
  67. [26] CToole, C. M., Povey, S., Hepburn, R, Franks, L. M:. Identity of some human
  68. bladder cancer cell lines. Nature. 1983, 301, 429-430.
  69. [27] Pletzold, J. L., Leigh, 1., Duffy, P. G., Masters, J. R. W.: Culture and charac-
  70. terization of human urothelium in vivo and in vitro. Urol. Res. 1994, 22: 67-74.
  71. [28] Sawicki, W-; Histologia, wyd. II. PZWL. Warszawa 1997, 93.
  72. [29] Scheidegger, G.: Der Aufbau des Ubwergangsepithels der Harnblase bei Schwein,
  73. Schaf Ratte und Spitznmaus. Acta Anat. 1980,107, 268-275.
  74. [30] Scheidegger, G., Ludwig, K. S.: Ultrastructural study of the transitional epithe-
  75. lium of the common. European white-toothed shrew (Crocidura russula). Experien.
  76. 1977, 33, 1507-1509.
  77. [31] Takami, Y., Matsuda, T., Yoshitaka, M., Hanumadass, M., Walter, J.:
  78. Dispase/detergent treated dermal matrix as dermal substitute. Burns. 1996,22,182-
  79. 190.
  80. [32] Tiltman, A. J., Friedell, G. H.: Effect of feedinf N-[4-(5-nitro-2-furyl)-2-
  81. thiazolyl]fornmamide on mitotic activity of rat uńnary-bladder epithelium. J. Nat.
  82. Cancer Inst. 1972, 48,125-129.
  83. [33] Wlodarski, K. H.: Bone formation in soft tissues, Bone (ed) Hall B. K., vol. 5,
  84. Fracture repair and regeneration. Boca Raton, CRC Press. 1992,313-337.
  85. [34] Wlodarski, K. H., Kuzaka, B., Wlodarski, R: Species differences in uroepithe-
  86. lium-induced bone deuelopment: obserations on transplants of human uroepitheli-
  87. um in cortisone-treated mice. Eur. Urol. 1995, 27,227-231.
  88. [35] Wlodarski, K. H., Kuzaka, B., Wlodarski, R K., Torz, C: Is heterotopic bone
  89. formation, occuring near a cut ureter, a sign of osteoinductive potency of human
  90. urothelium? Urol. Int. 1995, 55,115-117.
  91. [36] Wlodarski, K. H., Kuzaka, B., Wlodarski, P. K.: Human urinary bladder-
  92. carcinoma cells are nonosteoinductive. World J. Urol. 1996,14, S16-S20.
  93. [37] Yi, E. S., Shabiak, A. S., Lacey, D. L., Bedoya, A. A., Yin, S., Housley, R.
  94. M., Danilenko, D.M., Benson, W., Cohen, A. M., Pierce, G. F., Thomson,
  95. A., Ulich, T. R.: Keratinocyte growth factor causes proliferation of urothelium in
  96. vivo. J. Urol. 1995,154,1566-1570.