CODE: 3.1 - Uric acid plasma level and urine pH changes following ambroxol (ABX) parenteral treatment in experimental setting
Article published in Urologia Polska 2006/59/Suplement 1.

authors

Tomasz Drewa ¹, Zbigniew Wolski ¹, Bartosz Misterek ¹, Marzena Gruszka ², Celestyna Mila-Kierzenkowska ³

¹ Klinika Urologii Ogólnej, Onkologicznej i Dziecięcej, CM UMK w Bydgoszczy
² Zakład Diagnostyki i Laboratorium Szpitalne, CM UMK w Bydgoszczy
³ Zakład Inżynierii Tkankowej, CM UMK w Bydgoszczy

summary

Introduction. It was documented that ambroxol parenteral administration was associated with bladder stones in rats. Stones were composed of 67% of hypoxanthine and 33% of calcium oxalate.

Objectives. This study was undertaken to analyze the influence of selected factors on the stone formation after ABX parenteral administration.

Material and methods. 45 wild rats (Rattus sp.) were divided into 3 equal groups. Rats from the 1st and 2nd group received ABX s.c. during 2 weeks in total doses of 30 mg/kg/24h & 60 mg/kg/24h, respectively. Rats from the control group received 1 ml of injection solution s.c. During the ambroxol therapy rat urine was collected once daily during one month. To observe dynamics of pH changes after ambroxol administration 4 rats were treated parenterally for 10 days in dose of 60mg/kg/24h. Then rats were observed during the next two weeks, until the urine pH recovered to previous level. To establish the ambroxol influence on the purine metabolism the uric acid level was measured. 5 rats were treated with ambroxol parenterally (60mg/kg daily) for two weeks. Afterwards animals were sacrificed and 2 ml of blood form each was obtained. 5 rats were enrolled into the control group. Measurements were performed, using commercially available UA plus kit (Roche Diagnostics GmbH, Germany). Results were presented as means ? standard deviation. Student t-test was used to compare mean values, p <0.05 was considered as significant. Ethical committee permission was obtained.

Results. Rats urine pH increased in both treated groups (7.5 & 7.6) compared to the control group (6.7, p<0.05). There were no pH differences in both treated groups (p= 0.28). ABX withdrawal resulted in pH decrease to the previous value. In the control group plasma uric acid level was 5.7 ? 1.0 mg/dL. Plasma uric acid level increased up to 8.7 ? 1.0 mg/dL after two weeks of ABX treatment in dose of 60 mg/kg/24h (p<0.002).

Conclusions: ABX parenteral administration caused plasma uric acid elevation and increase of pH of rat urine. Both factors may be associated with hypoxanthine and oxalate stone formation after ABX parenteral administration.