COPPER AND ZINC LEVELS AND Cu/Zn RATIO IN SERUM
AND IN TISSUE OF RENAL CELL CARCINOMA IN HUMANS
Article published in Urologia Polska 2000/53/1.
authors
-
Zygmunt Dobrowolski 1, Tomasz Drewniak 1, T. Cichocki 2, Wojciech Kwiatek 3
- 1 Katedra i Klinika Urologii Collegium Medicum Uniwersytetu Jagiellońskiego
Kierownik: dr hab. med. Z. Dobrowolski
2 Katedra Histologii Collegium Medicum Uniwersytetu Jagiellońskiego
Kierownik: prof. dr hab. med. T. Cichocki
3 Instytut Fizyki J±drowej im. H. Niewodniczańskiego w Krakowie
Kierownik: dr hab. Z. Stachura
keywords
-
kidney RCC carcinogenesis Cu/Zn ratio
summary
- Introduction. At present, 25% to 40% of patients with Clinically localized renal
- cell carcinoma (RCC) have unpredictable recurrence after surgical therapy.
- Presently, no clinical serum marker exists to allow measurements of response
- to therapy in RCC. Significantly increased copper concentration and decreased
- zinc concentration in serum were found in diverse malignancies in humans
- lung and breast cancer, haematological malignancies
- in serum was io.und to be of great value in prognosis and therapy in these
- patients.
- Method. An SRIXE (Synchrotron Induced X-ray Emission) was used to measure
- concentration of the tracę elernents in this study. This physical analytical
- methods allows to defect concentrations about 10 ppb (partes per billion). Blood
- was collected from 15 patients before surgery. TNM classification was made in
- this group (T2- 4, T3- 7, T4- 4). The data were compared with concentration of
- copper and zinc in 8 healthy subjects. The levels of this tracę elernents were
- obtained from 36 cases of renal cell carcinoma. Results were compared with 15
- controls kidney cortex tissue obtained during autopsy in which cause of death
- was trauma. The non-parametric statistical analysis was used (Kruskal-Wallis,
- Friedman and McNemar statistical test).
- Results. Serum level of copper in RCC patients was significantly elevated with
- elevated ratio Cu/Zn as well. This increase was correlated with clinical TNM
- stage. The obtained copper and zinc levels in malignant tissue were significantly
- decreased. Correlation between Cu/Zn ratio in tissue and staging and grading
- was not found. The only statistically significant deference in concentration of
- the zinc was found between T4 and T2. There was no correlation in tracę elernents
- levels in tissue and serum in RCC patients.
- Conclusion. The abnormalities in copper and zinc metabolisms in malignancies
- was also found in RCC patients, but clinical importance re±uires further
- investigation in a bigger group of patients.
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