CORRELATION BETWEEN CYCLIN D1 EXPRESSION
AND RENAL CELL CANCER (RCC) PROGNOSTIC FACTORS.
INITIAL STUDY
Article published in Urologia Polska 2000/53/1.
authors
-
Sławomir Chaberek 1, Agnieszka D. Kolasińska 2, Kerstin Rolfe 2, Marek Żabiński 3, Danuta Shafie 3, Gidon Lieberman 2, Jarosław Ćwikła 1
- 1 Department of Radiology and Nuclear Medicine, SPSK 2, Otwock
Head of Department: Dr. J. Szawdyn
2 Academic Department of Obstetrics and Gynaecology Royal Free Hospital, London, UK
Head of Department: Prof. R. McLean
3 Department of Pathology and Urology, Departmental Hospital, Olsztyn
Head of Department: Dr. J. Gugała and Dr. M. Żabiński
keywords
-
kidney RCC cyclin D1 immunohistochemistry image analysis
summary
- Objective. In this initial study we have evaluated the correlation between
- cyclin D1 expression, morphological characteristics and outcome in patients
- with renal cell carcinoma (RCC).
- Patients and methods. Samples where obtained after total nephrectomy
- from 12 patients (mean age 57; SD 5. 43; male to female ratio 1: 1; age range
- 45-66) all with histological confirmation of RCC. Staining of cyclin D1 was
- evaluated using immunohistochemistry (IHC). A positive study was consid-
- ered if more than 20% ofpositive nuclei, were calculated both manually and
- automated. IHC results were compared to a range of RCC Prognostic factors
- age of patients, size of the tumour, TNM grading and Robson's score
- mean follow-up time was 30. 2 months (SD 7. 36).
- Results. The histological types were as follows: 8 clear cell, 2 granular cell
- and 2 papillary carcinomas. Cyclin D1 expression was detected in 9 patients
- Our findings were that there was no significant difference between cyclin D1
- expression and histological type of the tumour. There was no significant dif-
- ference between cyclin D1 expression and grading scales using TNM or Rob-
- son's classification. There was no correlation between cyclin D1expression
- and the above Prognostic factors of RCC. Finally, there was no significant
- difference between cyclin D1 expression in patients who died and those who
- survived (p = 0. 29).
- Conclusion. Analysis of cyclin D1 expression in RCC by IHC is a simple and
- easily reproducible technigue (manually and automated). Our initial results
- indicate that most RCC express cyclin Dr There was no correlation between
- cyclin D1 expression. and standard Prognostic factors of RCC.
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