mRNA, MDR-1 GENE EXPRESSION
IN RENAL CELL CARCINOMA PATIENTS. THE INITIAL STUDY
Article published in Urologia Polska 1997/50/4.
authors
-
Jarosław Ćwikła, Marek Żabiński 1, Sławomir D±browski 2, Leszek Królicki 3
- Zakład Roentgenodiagnostyki i Medycyny Nuklearnej
CMKP Warszawa, PSK nr 2 im. prof. Adama Grucy
Kierownik: lek. med. J. Szawdyn
1 Oddział Urologii
Zakład Opieki Zdrowotnej - Szpital Miejski im. Mikołaja Kopernika
10-045 Olsztyn, al. Niepodległo¶ci 44
Kierownik: lek. med. M. Żabiński
2 Katedra Mikrobiologii, Politechnika Gdańska
80-952 Gdańsk, ul. Narutowicza 11/12
Kierownik: prof. dr hab. J. Kur
3 Zakład Medycyny Nuklearnej i Rezonansu Magnetycznego
Wojewódzki Zespół Publicznych Zakładów Opieki Zdrowotnej
03-242 Warszawa, Kondratowicza 8
Kierownik: prof. dr hab. L. Królicki
keywords
-
kidney RCC multidrug resistance mRNA MDR-1, RT-PCR
summary
- Objective. The failure of chemotherapy to cure more than a minority of
- tumours is predominantly due to drug resistance. Cancers with overexpression
- of P-glycoprotein (Pgp) are resistant to a structurally and functionally diverse
- group of chemotherapeutic compounds which include anthracyclines, vinca
- alcaloids and actinomycin D. Pgp is encoded by MDR-1 gene. Normally high
- levels of mRNA MDR-1 gene expression was found in the normal human kidney,
- in the proximal tube epithelium. This tissue is usually an origin of most renal
- cell cancers. The renal cell carcinoma is a well-known tumour type demonstrating
- usually Pgp overexpression and usually it is resistant to standard chemotherapy.
- Some previous reports find out that overexpression of Pgp in renal tumours
- may be variable, most likely depending of tumour type and differentiation.
- Tumours reported to express no Pgp could be considered to appropriate
- chemotherapy. The aim of this initial study is estimation of mRNA MDR-1
- gene expression in renal cell carcinoma (RCC) patients.
- Patients and Methods. We considered in this study 10 patients with
- histopathological confirmation of RCC. All patients were treated by standard
- radical nephrectomy. Specimens of the tumour tissue were harvested during
- operation immediately after removed affected kidney. Each tumour samples
- were assessed to presence mRNA MDR-1 gene expression by specific semi-
- quantitative method, based on the reverse transcryptase ? polymerase chain
- reaction (RT-PCR).
- Results. The detectable level of MDR-1 expression was found in 5 patients.
- Others had no detectable levels.
- Conclusion. In this initial study we would like to presented a potential
- role of assessement mRNA MDR-1 gene expression measurement in untreated
- RCC patients. This simple and sensitive method seems to by promising tool
- in detecting MDR-1 gene expression and has a value in potential further
- chemotherapy in patients with RCC.
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