FLOW CYTOMETRY ANALYSIS OF DNA PLOIDY
IN BLADDER CANCER-RETROSPECTIVE ANALYSIS
Article published in Urologia Polska 1996/49/4.
authors
-
Anna Kołodziej, Danuta Du¶, Bogdana D±browska, Jerzy Lorenz
- Z Katedry i Kliniki Urologii AM we Wrocławiu
Kierownik Kliniki: prof. dr hab. med. Jerzy Lorenz
Z Oddziału Urologii Wojewódzkiego Szpitala Specjalistycznego we Wrocławiu
Ordynator: prof. dr hab. med. Jerzy Lorenz
Z Zakładu Immunologii Nowotworów Instytutu Immunologii i Terapii Do¶wiadczalnej PAN we Wrocławiu
keywords
-
bladder bladder cancer survival DNA ploidy
summary
- Flow cytometry analysis of DNA ploidy was performed on formalin fixed/paraffin-
- embedded tissue specimens obtained after transurethral tumor resection from 53
- patients. Follow-up period was 60 months.The DNA histogram were classified as
- diploid (22/53) and aneuploid (31/53) with one or more aneuploid cell population. A
- significant correlations were found; between T category and DNA ploidy - invasive
- tumors were mostly aneuploid, beetwen histopathological grade and ploidy, between
- type of growth and DNA ploidy. The results of DNA measurement were related to the
- clinical course of disease - patients with diploid tumors developed almost no local
- tumor progression for up to 5 years, whereas patients with aneuploid tumors in 65%
- suffered progression, metastatic disease and died. A measurement of proportion of S-
- phase cellls was not possible in half portion of specimens because poor quolity starting
- material (high CV., too much "debris") caused probably by poor quality starting material
- "heating effect" of electric loop or bad conditions of fixations and storing material
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