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FLOW CYTOMETRY ANALYSIS OF DNA PLOIDY IN BLADDER CANCER-RETROSPECTIVE ANALYSIS
Article published in Urologia Polska 1996/49/4.

authors

Anna Kołodziej, Danuta Du¶, Bogdana D±browska, Jerzy Lorenz
Z Katedry i Kliniki Urologii AM we Wrocławiu
Kierownik Kliniki: prof. dr hab. med. Jerzy Lorenz
Z Oddziału Urologii Wojewódzkiego Szpitala Specjalistycznego we Wrocławiu
Ordynator: prof. dr hab. med. Jerzy Lorenz
Z Zakładu Immunologii Nowotworów Instytutu Immunologii i Terapii Do¶wiadczalnej PAN we Wrocławiu

keywords

bladder bladder cancer survival DNA ploidy

summary

Flow cytometry analysis of DNA ploidy was performed on formalin fixed/paraffin-
embedded tissue specimens obtained after transurethral tumor resection from 53
patients. Follow-up period was 60 months.The DNA histogram were classified as
diploid (22/53) and aneuploid (31/53) with one or more aneuploid cell population. A
significant correlations were found; between T category and DNA ploidy - invasive
tumors were mostly aneuploid, beetwen histopathological grade and ploidy, between
type of growth and DNA ploidy. The results of DNA measurement were related to the
clinical course of disease - patients with diploid tumors developed almost no local
tumor progression for up to 5 years, whereas patients with aneuploid tumors in 65%
suffered progression, metastatic disease and died. A measurement of proportion of S-
phase cellls was not possible in half portion of specimens because poor quolity starting
material (high CV., too much "debris") caused probably by poor quality starting material
"heating effect" of electric loop or bad conditions of fixations and storing material

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