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Ligase chain reaction (LCR) used for detection of subclinical prostate infection caused by Chlamydia trachomatis
Article published in Urologia Polska 2005/58/1.

authors

Rościsław Bielecki 1, Bożena Zdrodowska-Stefanow 2, Iwona Ostaszewska-Puchalska 3, Marek Baltaziak 4, Małgorzata Skawrońska 5, Robert Kozłowski 1
1 Oddział Urologii SP ZOZ Wojewódzkiego Szpitala Zespolonego im. J. Śniadeckiego w Białymstoku
Ordynator oddziału: dr n. med. Robert Kozłowski
2 Klinika Dermatologii i Wenerologii Akademii Medycznej w Białymstoku
Kierownik kliniki: prof. dr hab. Bożena Chodynicka
3 Ośrodek Diagnostyczno-Badawczy Chorób Przenoszonych Drogą Płciową w Białymstoku
Kierownik ośrodka: dr n. med. Katarzyna Puciło
4 Zakład Patomorfologii Ogólnej Akademii Medycznej w Białymstoku
Kierownik zakładu: prof. dr hab. Andrzej Kemona
5 Zakład Medycyny Sšdowej Akademii Medycznej w Białymstoku
Kierownik zakładu: prof. dr hab. Jerzy Janica

keywords

prostate, Chlamydia trachomatis, prostatitis, ligase chain reaction, prostate biopsy

summary

introduction
Prostatitis is a common urological problem affecting men at every age. The evidence of bacterial infection could be demonstrated only in 5 to 10% of cases. Chlamydia trachomatis (Ch. trachomatis) infection is rarely diagnosed in chronic prostatitis.
aim of the study
Our goal was to analyze the frequency of subclinical Ch. trachomatis infection among sexually active men undergoing prostate biopsy due to prostate cancer suspicion.
material and methods
We evaluated 30 men in whom prostate cancer was excluded and evidence of prostatic inflammation was found in histology preparations. Sera samples were tested for anti-chlamydial IgG and anti-chlamydial IgA antibodies. The number of polymorphonuclear leukocytes (PMN) in urethral smears and expressed prostatic secretions (EPS) was assessed. Urethral smears and biopsy tissue specimens were tested for chlamydial DNA by ligase chain reaction (LCR) method. The NIH Chronic Prostatitis Symptom Index score was obtained for every patient.
results
Two out of 30 men were tested positive for chlamydial DNA in biopsy tissue specimens with LCR. None of them had positive history of prostatitis. Their sera samples were negative for anti-chlamydial antibodies. The inflammatory changes on histology preparations were estimated low and high according to CPCRN and IPCN classification. The urethral smears of both men and EPS of one were negative for PMN. The EPS of one patient was positive for PMN.
conclusion
In the case of asymptomatic prostatitis, the presence of subclinical Ch. trachomatis infection should be considered. Molecular methods could be helpful in the diagnostic process due to their high sensitivity and specificity.

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correspondence

Rościsław Bielecki
SP ZOZ Wojewódzki Szpital Zespolony im. Śniadeckiego
Oddział Urologii
ul. Skłodowskiej-Curie 26
15-960 Białystok
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