Diagnostic workup of the urinary tract diseases with the use of fluorescent probes and in situ hybridization techniques
Article published in Urologia Polska 2007/60/4.

authors

Tomasz Drewa, Zbigniew Wolski, Joanna Łysik: Katedra i Klinika Urologii Ogólnej, Onkologicznej i Dziecięcej, Collegium Medicum Uniwersytetu Mikołaja Kopernika w Bydgoszczy
Zakład Inżynierii Tkankowej, Katedra Biologii Medycznej, Collegium Medicum Uniwersytetu Mikołaja Kopernika w Bydgoszczy
Katedra i Klinika Onkologii i Brachyterapii, Collegium Medicum Uniwersytetu Mikołaja Kopernika w Bydgoszczy

keywords

bladder cancer, renal cancer, Wilms' tumor, prostate cancer, testicular cancer, infertility

summary

Introduction.The principles of fluorescence microscopy, DNA probe hybridization technique to the target place and fluorescence in situ hybridization (FISH) were presented in this review. Different FISH techniques were shown together with their potential applications.

Material and method. Medline was searched for the material for analysis. The applications of FISH in detection and diagnostic workup of the transitional cell carcinoma (TCC), prostate cancer, renal and testicular tumors and sexual differentiation together with infertility management were presented and discussed.

Results. The FISH technique will be applicable in TCC, prostate cancer and Wilms’ tumor diagnostic workup. The FISH technique can be used in the histological analysis of hybrid renal tumors. FISH analysis is nowadays wildly used in the research and cytogenetic studies in consulting on fertility.

Conclusions. The FISH technique is a time consuming procedure. Cellular slides should be prepared and sometimes in vitro culture is needed. The price of the single test is high. The above mentioned obstacles cause that process of introducing FISH technique into clinical practice is slow.

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