EVALUATION OF RENAL CELL CARCINOMA TUMORS ANGIOGENIC
ACTIVITY IN EXPERIMENTAL CUTANEOUS MODEL IN VIVO
Article published in Urologia Polska 2000/53/1.
authors
-
Krzysztof Pastewka 1, Ewa Skopińska-Różewska 2, Ewa Sommer 2, Mirosław Kazoń 1, Andrzej Borówka 1
- 1 Klinika Urologii CMKP w Warszawie
Kierownik: prof. dr hab. med. A. Borówka
2 Zakład Immunologii IGiCHP w Warszawie
Kierownik: prof, dr hab. med. E. Skopińska-Różewska
keywords
-
kidney RCC carcinogenesis angiogenesis
summary
- The aim of the study was to induce angiogenic activity of human renal cell
- carcinoma (RCC) tumors in Balb/C angiogenesis test.
- Material and method. The difference of angiogenic activity between cancer
- cells and phisiological Parker medium and between kidney tumors and normal
- kidney tissue homogenates by evaluation of activation ratio (Wp) were
- investigated. The experimental test of the in vivo mouse cutaneaus angiogenesis
- is valuable method for the assessment of neovascularisation induced by isolated
- neoplasmatic cells or tissue homogenates of renal cell carcinoma (RCC).
- The assessment of proangiogenic activity in dissection microscope is difficult
- as far as differentiation between newly formed blood yessels and background
- is concerned (standard of Sidky, Auerbach is obvious).
- Results. Injection of 5 x 105 cells per inoculation of RCC caused clear
- enhancement of angiogenesis Comparing to control electrolites Parker medium
- activation ratio, Wp = 4.47
- Comparing to normal renal tissue homogenates (Wp = 1.6).
- Conclusions. The authors observed in experimentalmodel in vivo high
- angiogenic activity of human RCC tumours.
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