THE EFFECT OF PIROXICAM ON ANGIOGENIC ACTMTY
OF KIDNEY AND URINARY BLADDER CANCER CELLS
Article published in Urologia Polska 1999/52/4.
authors
-
Ewa Sommer, Krzysztof Pastewka, Ewa Skopińska-Różewska, Małgorzata Filewska, Beata Białas-Chromiec, Mirosław Kazoń
- Zakład Immunologii Instytutu GruĽlicy i Chorób Płuc w Warszawie
Kierownik: prof. dr hab. E. Skopińska-Różewska
Klinika Urologii CMKP w Warszawie
Kierownik: prof. dr hab. A. Borówka
Zakład Patomorfologii CMKP w Warszawie
Kierownik: dr med. K. Bardadin
keywords
-
neoplasma non?steroidal antiinflammatory drugs ? Piroxicam ? angiogenesis ? antiangiogenic activity
summary
- Objective. Evidence of cancer chemopreventive potential of non-steroidal
- antiinflammatory drugs (NSAIDs) has recently been accumulated from
- experimental in vivo carcinogenesis and epidemiological studies. Although
- inhibition by NSAIDs of cyclooxygenase activity has been postulated to be
- involved in their cancer chemopreventive effects, the mechanism is not clear.
- Because tumour formation is strictly dependent upon neovascularisation we
- explored the possibility that inhibition of angiogenesis may contribute to
- antineoplastic properties of piroxicam.
- Material and methods. Tumour cells were isolated from 6 human kidney
- and 4 urinary bladder tumours removed during operation. We used the mouse
- cutaneous angiogenic test of Sidky and Auerbach, in our modification. The
- full suspension of tumour cells were grafted id. into Balb/c mice. Mice were
- then fed for 3 days with 0.02-0.4 mg/mouse piroxicam. Three days after cells
- grafting mice were sacrificed and newly-formed blood yessels were counted
- in dissection microscope.
- Results. Piroxicam significantly inhibited the angiogenic activity at doses
- 0.02-0.4 mg/mice.
- Conclusion. Piroxicam posses antiangiogenic activity.
references
- [1] Barenbaum, A., LeDenmat, R, Nevalainem, T., Masliah,J., Fournier, C:
- Interleukin-l-induced prostaglandyn E biosynthcsis in human synouialc cells invo-
- lves the actwation of' cytosoiic phospholipase A and cyclooxygenase-2. Eur. J. Bio-
- chem. 1994, 226, 125-131.
- [2] DuBois, M., Giardello, F., Smalley, W.: Nonsteroidal anti-inflammatory drugs,
- eicosanoids, and colorectal cancerprcvention. Gastroenterol. Clin. North. Am. 1996,
- 25, 773-791.
- [3] Hanif, R., Pittas, A., Feng, Y., Koutsos, M., Qiao, L., Staiano Coico, L.,
- Shiff, S. I., Rigas, B.: Effect of nonsteroidal anti-inflammatory drugs on prolifera-
- tion and on induction of apoptosis in colon cancer cells by a Prostaglandin-indepen-
- dent pathway. Biochem. Pharmacol. 1996, 52, 237-245.
- [4] Hubbard, W., Alley, M., McLemore, T., Boyd, M.: Profiles of Prostaglandin
- biosynthesis in sixteen established cell lines derived from human lung, colon, prostate
- and ovarian tumors. Canc. Res. 1998, 48, 4770-4775.
- [5] Kargmann, S., O'Neill, G., Vickers, P, Evans, J., Mancini, J., Jothy, S.:
- Expression of Prostaglandin G/H synthasel and 2 protein in human colon cancer.
- Cancer Res. 1995, 55, 2256-2259.
- [6] Moon, R., Kelloff, G., Detrisac, C, Steele, V., Thomas, C: Chemoprevention
- of OH-BBN-induced bladder cancer in mice by piroxicam. Carcinogenesis 1993,14,
- 1487-1489.
- [7] Noguchi, M., Eavashi, M., Minami, M., Kinoshita, K., Miyazaki, I.: Ef-
- fects of eicosapentaenoic acid on cell growth and Prostaglandin E and leukotriene B.
- production by a human breast cancer cell line (MDA-MB-231). Oncology 1995,52,
- 458-264.
- [8] Okajima, E., Ozono, S., Endo, T., Majima, T., Tsutsumi, M., Fukuda, T.,
- Akai, H., Denda A., Hirao, Y., Okajima, E., Nishino, H., Nir, Z.,
- Kon ishi, Y.: Chemopreven tive efficacy of piroxicam administered alone or in Combi-
- nation with lycopene and ?-carotene on the development of rat urinary bladder car-
- cinoma after N-butyl-N(4-hydroxybutyl) nitrosamine treatment. Japan. J. Cancer
- Res. 1997, 88, 543-552.
- [9] Quesada, C, Kimata, H., Mori, M., Nishimura, M., Tsuneyoshi, T.,
- Baba, S.: Piroxicam and acarbose are chemopreventive agents for spontaneous inter-
- stinal adenomas in APC gene 1309 knockout mice. Japan. J. Cancer Res. 1998,89,
- 392-396.
- [10] Rao, K., Detrisac, C, Steele, V., Hawk, E., Kelloff, G., Mc Comic, D.:
- Differential activity of aspirin, ketoprofen and sulindac as cancer chemopreventive
- agents in the mouse urinary bladder. Carcinogenesis 1996,17,1435-1438.
- [11] Reddy, B., Maruyama, H., Kelloff, G.: Dose-related inhibition of colon carcino-
- genesis by dietary piroxicam, a nonsteroidal antiinflammatory drug during different
- stages of rat colon tumor development. Canc. Res. 1987,47, 5340-5346.
- [12] Shibata, M., Hasegawa, R., Shivai, T., Takesada, Y., Fukushima, S.: Che-
- moprevention by indomethacin of tumor promotion in a rat urinary bladder carcino-
- genesis model. Inter. J. Cancer 1993, 55,1011-1017.
- [13] Shiff, S., Koutsos, M.I., Qiao, L., Rigas, B.: Nonsteroidal antiinflammatory
- drugs inhibit the proliferation of colon adenocarcinoma cells: effect on cell cycle and
- apoptosis. Exp. Cell. Res. 1996, 222,179-188.
- [14] Sidky, V. A., Auerbach, R.: Lymphocyte-induced angiogenesis: A quantitative
- and sensitive assay by modified assay of the graft-vs-host reaction. J. Exp. Med.
- 1975, 141, 1084.
- [15] Skopińska-Różewska, E., Sommer, E., Demkow, U., Chorostowska-Wy-
- nimko, J., Bałan B., Różycka, B., Barcz, E., Filewska, M., Mlekodaj, ¦.,
- Rudziński, R, Seweryniak, W., Caban, R, Kupis, W., Bogdan, ]., Usiek-
- niewicz, R, G±tarek, ]., Langfort, R.: Screening of angiogenesis inhibitors by
- modified tumor-induced angiogenesis (TIA) test in lung cancer. Ann. Acad. Med.
- Bialostocensis 1997, 42, (Suppl. 1), 287-296.
- [16] Skopińska-Różewska, E., Piazza, G. A., Sommer, E., Pamukcu, R., Barcz,
- E., Filewska, M., Kupis, W., Caban, R, Rudziński, R, Bogdan, ]., Mleko-
- daj, S. Sikorska, E.: Inhibition of angiogenesis by sulindac and its sulfone metabo-
- lite (FGN-1): A potential mechanism for their antineoplastic properties. Inter. J. Tiss.
- React. 1998, 20, 85-89.
- [17] Sommer, E.: Antynowotworowe dzałanie niesteroidowych leków przeciwzapalnych
- ze szczególnym uwzględnieniem Sulindaku. Terapia 1998, 7, 31-33.
- [18] Tanaka, T. Nishikawa, A., Mori, Y., Morishita, Y., Mori, H.: Inhibitory
- effects of nonsteroidal antiinflammatory drugs, Piroxicam and Indomethacin on 4-
- Nitroquinoline l-oxide-induced ton±ue carcinogenesis in male ACI/N rats. Canc.
- Lett. 1989, 48,177-182.
- [19] Thompson, H.; Briggs, S., Paranka, R, Piazza, G., Brendel, K., Gross, R,
- Sperl, G., Pamukcu, R., Ahnen, S.: Inhibition of mammary carcinogenesis in
- rats by sulfone metabolite of Sulindac. J. Natl. Cancer Inst. 1995,16,1259-1260.
- [20] Thun, M., Namboodini, M., Heath, C: Aspirin use and reduced risk offatal
- colon cancer. N. Engl. J. Med. 1991,325,1593-1596.
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