PTU - Polskie Towarzystwo Urologiczne
list of articles:

COMPARATIVE STUDIES OF THE INFLUENCE OF PERINATAL ESTROGENIZATION ON MORPHO-FUNCTIONAL STATUS OF THE ACCESSORY SEXUAL GLANDS IN MALE RAT
Article published in Urologia Polska 1998/51/4.

authors

Bolesław Otulakowski, Andrzej Limanowski 1, Bogdan Miskowiak 1
Katedra i Zakład Anatomii Prawidłowej AM w Poznaniu
Kierownik Zakładu: prof. dr hab. W. Woźniak
1 Katedra i Zakład Histologii i Embriologii AM w Poznaniu
Kierownik Zakładu: prof. dr hab. A. Łukaszyk

keywords

male sexual glands ? neonatal period ? estrogenisation

summary

Aim of the studies. The aim of these studies was to define the character
of changes induced in accessory sexual glands of male rats by a single injection
of estrogens on extrauterine day one and to compare the degree of sensitivity
of the investigated organs on induced hormonal disturbances.
Material and methods. The investigations were performed in male rats of
Wistar strain, injected s.c. with a single dose of 1 mg of stilboestrol
dipropionate (Polfa, Poland) on extrauterine day one. Three months after
the injection the animals were sacrificed under ether anaesthesia and testes,
epididymides, ventral prostate and seminal vesicles were examined
histologically. Serum testosterone was determinated by RIA. The relative
weight of the investigated organs and serum testosterone levels were
statistically evaluated by Duncan test.
Results. The estrogenization performed on day one of extrauterine life
resulted in a significant reduction of the relative weight of all the organs
tested. The testes of estrogenized animals reached 23.7%, epididymides 9.9%,
ventral prostate 8.2% and seminal vesicles merely 6.8% of the relative weight
of the control animals. Serum testosterone level in estrogenized animals
decreased to about 50% compared with the control. In all hwestigated glands
metaplasia of glandular epithelium into the multilayer and proliferation of
connective tissue-muscular stroma was evidenced. The most pronounced
changes were demonstrated in seminal vesicles, then in ventral prostate and
caput epididymis. The changes in the duct of epididymis were least prono-
unced.
Conclusions. On the basis of the results obtained it was concluded that
perinatal estrogenization leads to major morpho-physiological changes in
accessory sexual glands; the most profound changes beeing observed in seminal
vesicles, and the least profound in the duct of epididymis.

references

  1. [1] Arnold, A., Bredlove, S.: Organizational and activational effects of sex ste-
  2. roids on brain and behavior: a reanalysis. Horm. Behav. 1985, 19, 469-498.
  3. [2] Bartsch, G., Brungger, A., Schweikert, U., Hintner, H., Hopfl, R., Rohr,
  4. H.: Benign prostatic hyperplasia: a stromal disease. Urologe A 1989, 28, 321-328.
  5. [3] Cooke, P. S., Young, R, Hess, R. A., Cunhe, G. R.: Estrogen receptor
  6. expression in developing epididymis efferent ductules and other male reproduc-
  7. twe organs. Endocrinology 1991, 128, 2874-2879.
  8. [4] Corbier, P.: Sexual differentiation of positive feedback: effect of hour of castra-
  9. tion at birth on estradiol induced luteinizing hormone secretion in immature
  10. male rats. Endocrinology 1985, 116, 142-147.
  11. [5] Dalterio, S., Bartke, A., Steger, R., Mayfield, D.: Neonatal exposure to
  12. DES in BALB-c male mice: effect on pituitary-gonadal function. Biochem. Be-
  13. hav. 1985, 22, 1019-1024.
  14. [6] Gayton, F., Aceitero, I., Bellido, C, Pinilla, L., Aguilar, R., Aguilar,
  15. E.: Are cosinpophil leucocytes involved in the oestrogenic response of the post-
  16. natal rat epididymis. Int. J. Androl. 1990, 13, 500-507.
  17. [7] Handa, J. R., Corbier, P., Shryne, J. E., Schoomaker, J. N., Górski,
  18. R. A.: Differential effect of perinatal steroid environment on three dimorphic
  19. parameters on the rat brain. Biol. Reprod. 1985, 32, 855-870.
  20. [8] Hatier, R., Malaprade, D., Roux, M., Nguyen, B. L., Grignon G., Pa-
  21. sąualini, J. R.: Autoradiographic localization of 3H oestradiol in epididymis,
  22. seminal vesicles and prostate of the fetal guinea pig. Int. J. Androl. 1990, 13,
  23. 147-154.
  24. [9] Levine, A. C, Ren, M.: The effect of androgen, estrogen and growth factors
  25. on the proliferation of cultured fibroblasts derwed from human fetal and adult
  26. pwstates. Endocrinology 1992, 130, 2413-2419.
  27. [10] Limanowski, A., Miskowiak, B., Otulakowski, B.: Effect of estrogeniza-
  28. tion in the first day of life on the reproductine system in male rats. Histol. Hi-
  29. stopathol. 1994, 9, 59-63.
  30. [11] Limanowski, A., Otulakowski, B., Miskowiak, B., Filipiak, B.: Wpływ
  31. jednorazowego wprowadzenia stilbestrolu w 1. dniu życia na morfologię i czyn-
  32. ność gruczołu krokowego szczura. Urol. Pol. 1994, 47, 132-138.
  33. [12] Normand, T., Jean-Faucher, C, Jean, C: Neonatal exposure to estrogens
  34. alters the protein profiles and gene expression in the genital tract of adult male
  35. mice. J. Steroid Biochem. 1990, 5, 415-413.
  36. [13] Pinilla, L., Cocconi, M., Zopi, S., Martini, L.: Effect of neonatal estroge-
  37. nization and testosterone metabolism in the prostate and in the epididymis of
  38. the rat. J. Steroid Biochem. 1989, 3, 459-465.
  39. [14] Prins, G.: Neonatal estrogen exposure induces lobe-specific alterations in adult
  40. rat prostate androgen expression. Endocrinology 1992, 130, 3703-3714.
  41. [15] Suzuki, K., Inaba, S., Takauschi, H., Takezawa, Y., Fukabori, Y., Su-
  42. zuki, T., Imai, K., Yamanaka, H., Honma, S.: Endocrine environment of
  43. benign prostatic hyperplasia ? relationships of sex steroid hormone levels with
  44. age and size of the prostate. Nippon Hinyokika Gakkai Zasshi 1992, 83, 664-
  45. -671.
  46. [16] Tilley, W., Horsfall, D., Skinner, J., Henderson, D., Marshall, V.:
  47. Effect of pubertal development on estrogen receptor levels and stromal morpho-
  48. logy in the guinea pig prostate. Prostate 1989, 15, 195-210.
  49. [17] Toney, T. W., Danzo, B. J.: Estrogen and androgen regulation of protein syn-
  50. thesis by the immature rabbit epididymis. Endocrinology 1989, 1, 231-242.
  51. [18] West, N. B., Brenner, R. M.: Estrogen receptor in the ductuli efferentes, epi-
  52. didymis and testis of rhesus and cynomolgus macaaues. Biol. Reprod. 1990, 42,
  53. 533-538.
  54. [19] Zhao, G., Holterhus, R, Dammshauser, I., Hoffbauer, G., Aumuller,
  55. G.: Estrogen-induced morphological and immunohistochemical changes in stro-
  56. ma and epithelium of rat ventral prostate. Prostate 1992, 21, 183-199.