Isoflavones: natural compounds augmented the apoptosis-inducing potential of TRAIL
for prostate cancer chemoprevention
Article published in Urologia Polska 2008/61/Supl. 1.
Ewelina Szliszka, Bogdan Mazur, Grażyna Pietsz, Wojciech Król
- Katedra i Zakład Mikrobiologii i Immunologii w Zabrzu, ¦l±ski Uniwersytet Medyczny w Katowicach
Introduction. Chemoprevention is one of the most promising approaches in cancer research, in which natural or synthetic agents are used to prevent cancer in normal and high-risk populations. Epidemiologic data suggested that consumption of products rich in isoflavones may protect against prostate cancer. In vitro study suggests that dietary isoflavones inhibit the proliferation of prostate cancer cells and tumor growth in animal models.
Objectives. In this study we investigated the combined effect of isoflavones and tumor necrosis factor related apoptosis inducing ligand (TRAIL) on prostate cancer cells.
Materials and methods. Human prostate cancer cell line LNCaP was incubated with TRAIL in the presence or absence of seven isoflavones: genistein, daidzein, biochanin A, puerarin, psoralidin, neobavaisoflavone, ipriflavone. Cytotoxicity was determined by MTT and LDH assays. Apoptosis was determined by flow cytometry (Apoptest-FITS test).
Results. Our study confirmed that LNCaP cells were resistant to TRAIL. We therefore examined the cytotoxic and apoptotic effect of isoflavones in combination with TRAIL on prostate cancer cells. Here it was found that co-treatment of LNCaP cells with noncytotoxic concentration of isoflavones significantly sensititizes prostate cancer cells to TRAIL induced apoptosis. Isoflavones markedly augmented TRAIL mediated cytotoxicity against LNCaP.
Conclusions. The obtained results suggest that combined treatment with TRAIL and isoflavones may provide the basis for a new chemoprevention approach to induce apoptosis in prostate cancer cells.