Tomasz Drewa, Victoria Sarafian, Zbigniew Wolski, Romana Joachimiak, Jan Sir, Anna Kaznica, Joanna Wisniewska-Skopinska5
- Katedra i Klinika Urologii Ogólnej, Onkologicznej i Dziecięcej CM w Bydgoszczy UMK w Toruniu
Katedra i Zakład Biologii Medycznej, Uniwersytet Plovdiv, Bułgaria
Zakład Inżynierii Tkankowej, Katedra Biologii Medycznej, CM UMK w Bydgoszczy
Zakład Patologii, Centrum Onkologii w Bydgoszczy
Katedra i Zakład Chemii Ogólnej, UMK w Toruniu
Introduction. It was proved that it is possible to construct a tissue-engineered bladder wall replacement with autologous cells obtained from non-urinary tract components.
Objectives. The aim of this study was to show in vitro construction of whole bladder wall graft from cells and scaffold, both obtained from non-urological components.
Materials and methods. Rat hair follicles epithelial cells and rat urothelial cells were both cultivated in DMEM (Sigma) supplemented with 10% of Fetal Bovine Serum (FBS) and EGF(10 ng/ml; Sigma). Cells were stained using anty-cytokeratine (CloneMMF) and anty-CD34 antibodies (Dako, Denmark). Collagen scaffold was prepared from tendons of Wistar rats tails. 6-well plates were covered with collagen scaffold. 15x103 of hair follicles epithelial cells and urothelial cells were seeded on collagen surface and cultured for a week. Cells in the controls were seeded on polystyrene surface of 6-well plates. After a week cell viability was assessed using MTT test (Sigma) and photo
- documentation was prepared.
Results. Hair follicles epithelial cells expressed epithelial markers and were slightly positive for CD34. Hair follicles epithelial cells proliferated very quickly. There were 292.5 ±33.3 x 103 and 167.4 ±24.9 x 103 of cells growing on polystyrene and collagen, respectively. Urothelial cells expressed epithelial markers and were negative for CD34. These cells proliferated slowly. There were 40.0 ±4.2x103 and 4.5 ±1.8 x 103 cells growing on polystyrene and collagen, respectively.
Conclusions. Hair follicles progenitor cells can be potentially used in tissue-engineering, with the guarantee of the sufficient cell number for transplantation. Collagen scaffold slowed cell proliferation and probably influenced on cell differentiation process. It seems that the construction of the bladder wall from cells and scaffold coming from non-urological tissues is possible.