Cytotoxic activity of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) against bladder cancer cells after using chemotherapeutic drugs
Article published in Urologia Polska 2007/60/2.

authors

Ewelina Szliszka, Anatol Majcher, Maciej Domino, Grażyna Pietsz, Wojciech Król: Katedra i Zakład Mikrobiologii i Immunologii w Zabrzu Śląskiej Akademii Medycznej w Katowicach

keywords

urinary bladder, bladder cancer, TRAIL, chemotherapeutic drugs

summary

Introduction. Transitional cell carcinoma (TCC) of the bladder is a common malignancy. The majority of bladder tumors are superficial at diagnosis and, after local surgical therapy, have a high rate of local recurrence and progression. Current treatments extend the time of recurrence but do not alter disease survival rate. Tumor necrosis factor related apoptosis in-ducing ligand (TRAIL) belongs to the tumor necrosis factor superfamily and it selectively induces apoptosis in cancer cells, with no toxicity against normal tissues.

The aim of the study. TRAIL is one of the most promising candidates for cancer therapy. In this study, we investigated the effect of TRAIL on bladder cancer cells with or without che-motherapeutic drugs pretreatment.

Material and methods. Human transitional bladder cancer cell line SW780 was tested for TRAIL sensitivity in the presence or absence of the chemotherapeutic agents: doxorubicin, epirubicin, gemcitabine and mitomycin C. Cytotoxicity was determined by MTT and LDH assays.

Results. We reported that chemotherapeutic agents significantly augmented TRAIL mediated cytotoxicity against transitional bladder cancer cells. The most potent cytotoxic effect in com-bination with TRAIL was exhibited by doxorubicin (85,9 +/-1,0 % killing).

Conclusion. The increase of cytotoxic effect of TRAIL after application of low dose chemo-therapeutic agent in bladder cancer cells was observed. This study demonstrated that TRAIL treatment combined with chemotherapy may provide the basis for a new therapeutic approach to induce apoptosis in bladder cancer cells and decrease the dose of chemotherapeutic drugs.

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