ESTROGEN AND PROGESTERON RECEPTORS
IN PARATESTICULAR TISSUES IN CRYPTORCHID BOYS.
PRELIMINARY RAPORT
Article published in Urologia Polska 1999/52/4.
authors
-
Przemysław Przewratil, Józef Kobos, Jerzy Niedzielski
- Klinika Chirurgii i Onkologii Dziecięcej IP AM w Łodzi
Kierownik: dr n. med. J. Niedzielski
Pracownia Patomorfologii Instytutu Pediatrii AM w Łodzi
Kierownik: dr hab. med. J. Kobos
keywords
-
testis cryptorchidism estrogen and progesteron receptors
summary
- Cryptorchidism is the serious developmental disorder which point is stop
- the testis on its way of descent to the scrotum. 2-8% of all male newborns are
- afected with this defect. It re±uires early hormonal therapy or/and surgery.
- Mechanism of maldescent is still not well recognized. There are two probable
- pathologic mechanisms: anatomical and hormonal. Second one is based on
- disruptions in the hypothalamus-pituyitary-testis axis. There are many data
- on the role of androgens in testicular descent but the role of estrogens in this
- process is not well known. The aim of this study is to explain the role of estrogens
- and progesteron and their tissular receptors in pathogenesis of cryptorchidism.
- Material and method. 19 patients (48 specimens) with unilateral crypto-
- rchidism, aged from 2 to 8 years were analysed. In all cases during the orchi-
- dopexy, specimens of gubernaculum, cremaster muscle and processus vaginalis
- were taken. 17 boys (25 specimens), aged from 1 to 6 years, operated on due
- to inquinal hernia, with testes in scrotum formed control group. Histo-
- pathological specimens normaly prepared were stained with hematoxyline
- and eosine (H+E) and tested immunohistochemicaly in LSAB detecting system,
- according to immunoperoxidase method. Monoclonal mice antybody against
- Estrogen Receptor (ER) and Progesterone Receptor (PR) was used. Histo-
- pathological structure and then receptor immunoreactivity was assessed in
- each specimen.
- Results. Cryptorchid group - ER was detected in 9 (fraction 0.6) cases
- and PR in 7 (fraction 0.47) cases of 15 gubernaculum specimens. ER was detected
- in 18 (fraction 0.95) cases and PR in 14 (fraction 0.74) cases of 19 cremaster
- specimens. ER was detected in 11 (fraction 0.79) cases and PR in 6 (fraction
- 0.43) of 14 processus vaginalis specimens. Control group - ER and PR were not
- detected in any of 10 cremaster specimens. ER was found only in 2 (fraction
- 0.13) cases and PR in 1 cases (fraction 0.07) of 15 processus vaginalis specimens.
- In both cases there were single cells with receptor immunoreactivity. According
- to these results it is clear that estrogen and progesteron receptors are present
- in most cases in cremaster and processus vaginalis of cryptorchid children. In
- control group this expression was not found. In gubernaculum hormonal
- receptors are also present but in lesser degree.
- Conclusion. Except of androgen effect there is probable significant
- correlation between disruptions in estrogen regulation, expressed in tissues
- receptors, and testicular maldescent. Obtained results can be a proof of fetal
- exposition to estrogens in tested patients.
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